Background: Public health initiatives are crucial for disease prevention and improving population health outcomes. However, the effectiveness of health education programs in diverse cultural contexts remains underexplored. Objective: This study aims to evaluate the impact of health education on disease prevention across three continents, focusing on varying cultural and socio-economic settings. Methods: A mixed-methods approach was employed, involving surveys and focus group discussions with participants from Austria, Ghana, and Mexico. Quantitative data were analyzed using statistical software to identify trends in health behavior changes, while qualitative data provided insights into cultural perceptions of health education. Findings: The results indicate a significant improvement in awareness and preventive behaviors post-intervention, with variations observed across regions. European participants showed the highest increase in knowledge retention, while African participants reported the greatest change in proactive health behaviors. Mexican participants highlighted the importance of culturally tailored messaging for effective health education. Conclusion: Health education programs are effective in promoting disease prevention, with cultural adaptation enhancing their impact. These findings underscore the need for region-specific strategies in public health planning and policy formulation, aimed at maximizing educational outreach and disease prevention in diverse populations.